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SM9000光谱仪是一种高分辨率光纤光谱测量仪,测量范围涵盖紫外光、可见光乃至近红外波段。SM9000既可以单独使用,也可以与FKM多光谱荧光动态显微成像系统、FL3500双调制叶绿素荧光仪等仪器联用,测量各种荧光的光谱组成。由于其具备超高的灵敏度,甚至可以测量单个细胞激发荧光的光谱。每秒可记录100组16bits分辨率的光谱数据。
功能特点:
·超高灵敏度,可检测单个细胞的荧光光谱
·超高分辨率,可检测10μs - 10ms的闪光
·采集频率达100次/秒,可检测动态光谱
·积分时间从1毫秒到数分钟可调
·模块化设计,小巧耐用,热稳定性高
·产热量极低
技术参数:
·光谱范围:200 - 980nm
·分辨率:可检测10μs - 10ms的闪光
·采集频率:100次/秒
·积分时间:1毫秒到数分钟可调
·光学入口:直径0.5,数值孔径(NA)=0.22,可拆卸SMA接头
·入射狭缝:70µm×1400µm
·光栅:平场型校正
·波长精确度:< 0.5nm
·再现性:< 0.1nm
·温度漂移:< 0.01nm/K
·像素光谱距离:0.8nm
·FWHM半高宽:3 - 4nm
·杂散光:0.1%(氙灯340nm测量)
·CCD阵列像素数:1044×64
·像素尺寸:24×24mm2
·系统数据:16Bit模数转换
·可联用仪器:FKM多光谱荧光动态显微成像系统、FL3500双调制叶绿素荧光仪等
与FKM多光谱荧光动态显微成像系统联用的SM9000
应用案例:
与FKM系统联用研究铜指示植物海州香薷Elsholtzia splendens的叶绿素荧光及其光谱组成(Peng,2013,Environ. Sci. Technol)
与FL3500系统联用研究蓝隐藻Guillardia theta的叶绿素荧光及其光谱组成(Cheregi,2015,Journal of Experimental Botany)
产地:欧洲
参考文献:
1. Bernát G, et al. 2017. On the origin of the slow M–T chlorophyll a luorescence decline in cyanobacteria: interplay of short-term light-responses. Photosynthesis Research, DOI 10.1007/s11120-017-0458-8
2. Selyanin V, et al. 2016. The variability of light-harvesting complexes in aerobic anoxygenic phototrophs. Photosynthesis research, 128(1): 35-43
3. Tilstone G, et al. 2016. Effect of CO2 enrichment on phytoplankton photosynthesis in the North Atlantic sub-tropical gyre. Progress in Oceanography, 158: 76-89
4. Mishra K B, et al. 2016. Plant phenotyping: a perspective. Indian Journal of Plant Physiology, 21(4): 514-527
5. Cheregi O, Kotabová E, Prášil O, et al. 2015. Presence of state transitions in the cryptophyte alga Guillardia theta . Journal of Experimental Botany, 66: 6461-6470
6. Li G, Brown C M, Jeans J A, et al. 2015. The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytologist, 205:533-543
7. Kotabová E, Jarešová J, Kaňa R, et al. 2014. Novel type of red-shifted chlorophyll a antenna complex from Chromera velia. I. Physiological relevance and functional connection to photosystems. Biochimica et Biophysica Acta – Bioenergetics, 1837:734-743
8. Šebela D, Olejníčková J, Sotolář R, et al. 2014. The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA , 1817: 1237-1247
9. Peng H, et al. 2013. Toxicity and Deficiency of Copper in Elsholtzia splendens Affect Photosynthesis Biophysics, Pigments and Metal Accumulation. Environ. Sci. Technol., 47 (12): 6120-6128